Lukas Stühn

Lukas Stühn, M.Sc.

+49 6151 16-21922

Alarich-Weiss-Straße 16
64287 Darmstadt

Office: L2|07 206

Research fields

  • Multimodal atomic force microscopy in amplitude- and frequency-modulation mode and spectroscopy
  • Cultivation of human cell lines
  • Spincoating of polymeric thin films and embedding of nanoparticles
  • Three-dimensional nanomechanical characterisation and imaging of biological materials and polymers

PhD project

„Subsurface Imaging“ of magnetic nanoparticles and quantification of nanomechanical properties of polymers and biological materials by atomic force microscopy

Publications

3 Appel, Christian ; Kuttich, Björn ; Stühn, Lukas ; Stark, Robert W. ; Bernd Stühn
Structural Properties and Magnetic Ordering in 2D Polymer Nanocomposites: Existence of Long Magnetic Dipolar Chains in ZeroField
[Online-Edition: https://doi.org/10.1021/acs.langmuir.9b02094]
In: Langmuir, ISSN 0743-7463, (2019)
2 Stühn, Lukas ; Fritschen, Anna ; Choy, Joseph ; Dehnert, Martin ; Dietz, Christian:
Nanomechanical sub-surface mapping of living biological cells by force microscopy.
[Online-Edition: https://doi.org/10.1039/C9NR03497H]
In: Nanoscale, ISSN 2040-3364, (2019)
1 Grefe, Ann-Kathrin; Kuttich, Björn; Stühn, Lukas; Stark, Robert; Stühn, Bernd:
Oriented crystallization of PEG induced by confinement in cylindrical nanopores: Structural and thermal properties.
[Online-Edition: https://doi.org/10.1039/C9SM00053D]
In: Soft Matter ISSN 1744-683X [article], (2019)

Conference talks

2 Lukas Stühn, Anna Fritschen und Christian Dietz
“Nanomechanical sub-surface mapping of cells by atomic force microscopy”
21st annual Linz winter workshop – Advances in single-molecule research for biology and nanoscience, Linz, Feb. 2019
1 Lukas Stühn and Christian Dietz
“Subsurface Imaging” of magnetic nanoparticles and quantification of nanomechanical properties of polymers and biological materials by bimodal atomic force microscopy.
Annual meeting of the german society for biomaterials (DGBM), Würzburg, Nov. 2017 (upgraded poster)

Poster

3 Lukas Stühn, Anna Fritschen and Christian Dietz
Nanomechanical sub-surface mapping of cells by atomic force microscopy
DPG Spring Meeting of the Condensed Matter Section, Mar. 2019, Regensburg
2 Lukas Stühn, Julia Auernhammer and Christian Dietz
In situ observation of the pH-dependent dis- and reassembly process of ferritin nanoparticles by atomic force microscopy
7th Multifrequency AFM Conference, Apr. 2018, Madrid
1 Lukas Stühn, Julia Auernhammer, Anna Fritschen, Felix Nagler and Christian Dietz
Subsurface imaging of magnetic nanoparticlesand measurement of nanomechanicalproperties of polymers and biologicalmaterials by bimodal atomic force microscopy
DPG Spring Meeting of the Condensed Matter Section, Mar. 2018, Berlin

Teaching

  • “Fortgeschrittenenpraktikum II” (materials science department)
  • “Ferien im Labor” (materials science department)
  • “Erfinderlabor” (materials science department and centre for chemistry)
  • Mentoring of bachelor- and master-students

Description of project

Magnetic force microscopy does not only enable the detection of magnetic nanoparticles but also the detection of their position beneath the sample surface that can be combined with the recording of the mechanical properties of the overlying substance. This is made possible by by the bimodal excitation of the cantilever. Such measurements can be performed in various environments, e.g., liquids, with a high imaging rate. This tool facilitates the detection of, for example, diffusion processes of functionalised nanoparticles in human cells. In targeted drug delivery. the movement of active substance carriers is not entirely understood. Objective of this project is the establishment of a correlation between the favoured intruding sites of the nanoparticles with the mechanical properties of endothelial cells.

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