Asma Siddique

Asma Siddique, M.Phil.

+49 6151 16-21928
+49 6151 16-21921

Alarich-Weiss-Straße 16
64287 Darmstadt

Office: L2|07 203

Research fields

  • Biocompatible polymers
  • Surface functionalisation
  • Cell-surface interfaces
  • Mimicking vascular microenvironment at high shear stress
  • Mammalian, fungal, yeast, bacterial cell culture

PhD project

Surface modification of PDMS based microfluidic devices for improved cell adhesion under high shear stress

Publications

6 Siddique, Asma ; Meckel, Tobias ; Stark, Robert W. ; Narayan, Suman :
Improved cell adhesion under shear stress in PDMS microfluidic devices.
[Online-Edition: http://dx.doi.org/10.1016/j.colsurfb.2016.11.011]
In: Colloids and Surfaces B-Biointerfaces, 150 S. 456-464. ISSN 0927-7765
[article] , (2017)
5 Siddique, Asma ; Gul, Ambreen ; Irfan, Muhammad ; Nadeem, Muhammad ; Syed, Quratulain
Comparison of different pretreatment methods for efficientconversion of bagasse into ethanol
[Online-Edition: DOI: 10.1080/17597269.2016.1215066]
In: Biofuels, 8:1, 135-141
[Artikel], (2017)
4 Siddique, Asma ; Bashir, Aftab ; Latif, Farooq
Exploration of unique relationamong industrial fungi by statistical analysis
In: Songklanakarin J. Sci. Technol. 34(6), 607-613
[Artikel], (2012)
3 Khan, S.A. ; Siddique, A. ; Latif, F.
Fibrolytic enzyme production fromHumicola insolens and its applications in animal feed
In: Pakistan J. Zool. Suppl.Ser. 9:41-45
[Artikel], (2009)
2 Anwar, Farooq ; Siddique, Asma ; Iqbal, Shahid ; Asi, M. Rafique
Stabilization of sunflower oil with Moringa oleifera leaves under ambient storage
In: Journal of Food Lipids, 14: 35-49
[Artikel], (2007)
1 Siddique, A. ; Anwar, F. ; Manzoor, M. ; Fatima, A
Antioxidant activity ofdifferent solvent extracts of Moringa oleifera leaves under accelerated storage of Sunflower oil
In: Asian Journal of Plant Science, 4(6): 630-635
[Artikel], (2005)

Conference talks

2 Asma Siddique, Isabelle Pause, Suman Narayan and Robert W. Stark
Stability of protein coatings inside microfluidic devices under flow conditions
µFlu’18-NEGF’18, Feb. 2018, Strasbourg, France
1 Asma Siddique and Robert W. Stark
Microfluidics; A competent analytical tool in life science
Emerging trends in life sciences for sustainable development, Oct. 2014, Lahore Pakistan

Poster

3 Asma Siddique, Isabelle Pause, Suman Narayan and Robert W. Stark
Stability of protein coatings inside microfluidic devices under flow conditions
µFlu’18-NEGF’18, Feb. 2018, Strasbourg, France
2 Asma Siddique, Suman Narayan and Robert W. Stark
Stable protein coating inside PDMS microfluidic devices for improved cell adhesion under shear stress
Lab-on-Chip & Microfluidics 2017, May 2017, Munich, Germany
1 Asma Siddique, Suman Narayan and Robert W. Stark
Stable protein coating inside PDMS microchannels for cell growth under flow conditions
EMBL Conference, Microfluidics 2012, Jul. 2014, EMBL Germany

Teaching

  • “Fortgeschrittenenpraktikum II” (materials science department)
  • Mentoring of bachelor- and master-students

Description of project

Many of the vascular functions, hemodynamic forces, cellular interactions and related diseases are strictly related to of physical geometry of vasculature. Combination of microfluidics and cell biology offers a physiologically realistic platform to reproduce various vascular models in laboratory leading to better understandings about cell and tissue physiology in 3D environment. Under a shear flow, however, the stability of cells that were grown on physically adsorbed proteins is short lived, which limits long-term cell studies. Focusing on this issue various protein-substrate linkages were developed for stable cell growth under high flow conditions (at physiological shear stress in human vasculature). APTES (3-Aminopropyl)triethoxysilane is primarily used to immobilized collagen on PDMS surface for stable endothelial cells growth at long term, high shear stress.

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